His

0atx f J ALA

Oval o1LE

w LEU

Qser

O cys2

Ohyp

Figure 10.17 Two-dimensional high voltage electrophoresis and chromatography of amino acids. Paper high voltage electrophoresis (4000 V) in an acetic acid-formic acid buffer at pH 2.0 in the first dimension followed by descending chromatography in the second dimension in an n-butanol-acetic acid-water solvent (12:3:5). The spots were visualized with a ninhydrin-collidine reagent.

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