Isoelectric focusing

Electrophoresis depends primarily upon the charge carried by a molecule, but at its iso-electric pH (p/) a molecule will not migrate in an electric field. If electrophoresis is performed in an electrolyte that has a pH gradient, molecules will migrate to the point where the pH of the solution is the same as their iso-electric pH and remain there as long as the gradient and potential differences are maintained. This is the basis of iso-electric focusing techniques (Figure 3.28).

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