Since the publication of the first edition in 1983, several specialist books which cover a range of specific techniques in detail have been published. However, the ability to select an appropriate technique for a particular analytical problem still remains fundamental and the first edition of this book evidently proved useful in this respect. Thus the principal objective for this second edition remains unchanged.
Much of the information has been updated for the second edition to reflect substantial changes in the subject. The edition of a chapter on nucleic acids was considered essential and complements the original chapters on the chemical nature and methods of analysis of other important biological molecules. We are indebted to Dr Susan Laird for compiling this chapter and also to Mr Robert Smith for the major update on immunoassays in the immunological methods chapter.
We have maintained the same balance of information in the new chapter and therefore details of specific applications of techniques are not discussed, for example, DNA fingerprinting. Where appropriate, we have included titles of books which have an emphasis on applications in the further reading list at the end of each chapter. These lists are not intended to be fully comprehensive, nor are the chapters referenced as we consider this to be inappropriate for the level of potential readership.
We have received many pleasing reports of the usefulness of the first edition in a range of analytical laboratories, in areas such as pharmaceuticals, biotechnology, agrochemicals, clinical biochemistry, molecular biology, etc. Our own experience and comments from colleagues in other universities have reinforced our initial purpose of writing a book for students on a range of courses that include the analytical aspects of biochemistry. We are therefore delighted that this softback edition is now available which will encourage wider access for student use.
Hazel Peck David Holme
Sheffield Hallam University July 1992
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