CerbB2

The HER-2/neu (c-erbB-2) oncogene encodes a transmembrane tyrosine kinase receptor with extensive homology to the epidermal growth factor (EGF) receptor (28). Overexpression of the c-erbB-2 gene in human adenocarcinomas such as breast cancer or non-small cell lung cancer is thought to be associated with prognosis (29). The relationship between expression of erbB-2 and drug resistance in cancer has not been elucidated.

The KATOIII and MKN7 cell lines expressed the c-erbB-2 gene but not SW480. The introduction of AS-ODNs (erbB-2-AS) into KATOIII and MKN7 cells decreased the expression of c-erbB-2, but that of sense oligonucleotides (erbB-2-S) had no effect. An antisense and a control were transferred to the cells. The intensity of the fluorescent peaks in the cells treated with antisense was shifted to the left. This shift was observed in the KATOIII and MKN7 lines. We then tested the effect of AS-ODNs for c-erbB-2 on cisplatin sensitivity. All of four independent tests showed a severalfold increase in resistance to

Table 5

Inhibition of Drug Sensitivity (IC50) by c-erbB-2 AS-ODNs on Cellsa

Inhibition of Drug Sensitivity (IC50) by c-erbB-2 AS-ODNs on Cellsa

Table 5

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