CYP1A11A2 Activity

CYP1A1/1A2 activity was determined in the liver S9 fraction (postmitochondrial supernatant of tissue homogenate) by ethoxyresorufin-O-dealkylation assay (63). A 1-mg aliquot of S9 protein, 2 ^M 5-ethoxyresorufin (Pierce, Rockford, IL), and 1 mM ß-NADPH in a volume of 1 mL of potassium phosphate buffer (pH 7.4) was incubated for 10 min at 37°C. Samples were analyzed spectrofluorometrically using an excitation wavelength of 530 nm and emission wavelength of 585 nm. Concentrations of unknowns were calculated from a standard curve of resorufin (Pierce). Results were recorded in picomoles of resorufin per milligram of S9 protein per minute.

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