Assays of caspase activity

The demonstration that certain polypeptides (including procaspases) have been cleaved to their signature fragments (Tables 1 and 2) provides presumptive evidence that caspases might have been activated. Nonetheless, the possibility that other proteases could catalyse cleavage of a particular polypeptide to fragments of similar size must always be kept in mind. Accordingly, investigators commonly bolster the argument that caspases have been activated by assaying directly for caspase activity.

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