Equipment

The basic equipment (38, 39) consists of filtration funnels (25 mm polyethylene filter holder, Swinnex, Millipore Corp.) connected and cemented with epoxy to a 50 ml polyethylene syringe (Swinnex funnel). The syringe orifice should be enlarged with a 0.125 in (3.2 mm) diameter drill to facilitate filling the upper section of the filter without trapping air. The bottom of the filter holder is connected to a 15 gauge stainless steel needle inserted through a rubber stopper to fit a filtration funnel holder (Figure 1). A set of elution funnels is mounted on a support, with a rack under this support to accommodate liquid scintillation vials (Figure 1).

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