Initial studies on caspase and granzyme inhibitors were done in vitro, using purified molecules and cell-free systems. The validation of the results obtained concerning the possible implication of several caspases and/or granzymes in cell-mediated cytotoxicity should come using whole-cell assays. Several peculiarities of the inhibitors and of the assays should be kept in mind when performing these studies, which we will briefly consider now.
Editors note: Additional cautions regarding the use of caspase inhibitors are discussed in Chapter 10, Section 8.2.
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