Laurdan Probe the Tips

Generalized Polarization Laurdan

As is the case with the vast majority of UV-excited fluorescent probes, confocal or epifluorescence microscopy experiments are difficult to perform with LAURDAN. As already mentioned, the extent of LAURDAN photobleaching under epifluorescence and confocal microscopy is severe, making it almost impossible to collect images on LAURDAN-labeled specimens for more than a few seconds Bagatolli and Gratton 2001 . A way to circumvent this problem is by using two-photon-excitation fluorescence...