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Fig.9.7. GP Image of a living macrophage, 3D-reconstructed pseudocolored GP Images of RAW264.7cell (Image plane parallel to cover slip, viewed from above). Notice the different discrete GP regions on the cell membrane Indicating membrane lateral heterogeneity. (Adapted from Gaus et al. 2003, copyright 2003 National Academy of Sciences, USA)

plex situations. As discussed, this last type of information renders multiphoton-ex-citation microscopy a powerful tool to learn about similar phenomena in complex systems such as biological membranes and tissues.

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