Flumadine See rimantadine hydrochloride

fluorescein isothiocyanate (FITC) A fluorescent compound used for labeling proteins or nucleic acids. It is excited by light of wavelength in the range 450-490nm and emits in the range 520-560nm.

fluorescent antibody An antibody which is labeled with a fluorescent dye, e.g. fluo-rescein isothiocyanate. It can then be used in conjunction with a fluorescence microscope to detect viral antigens.

fluorescent immunoassay A useful approach to virus diagnosis, especially when virus-infected cells are available. Specific antibodies (e.g. monoclonal antibodies) are conjugated with fluorochrome dyes (e.g. fluorescein isothiocyanate) and used to detect viral antigens. Virus-infected cells are seen by microscopic examination. A similar approach can be used to detect specific antibodies in the host.

fluorodeoxyuridine A pyrimidine analog and a reversible inhibitor of DNA synthesis. Phosphorylated by cellular thymidine kinase and thus becomes an analog of thymidylic acid. Brings about chromosome breaks. Synonym: floxuridine.

fluorography A technique to detect 3H-labeled molecules in chromatograms and polyacrylamide gels. The scintillator

2,5-diphenyloxazole (PPO) is introduced into the chromatogram or gel which is then exposed to photographic film.

Laskey RA and Mills AD (1975) Eur J Biochem 56, 335

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