Paul BunnellDavidsohn test

Paul-Bunnell test for glandular fever as originally described consisted of a simple titration of the patient's serum for sheep erythrocyte-agglutinating antibodies. In order to distinguish between infectious mononucleosis, Forssman and serum-sickness antibodies (all of which agglutinate sheep cells), Davidsohn introduced an absorption step into the test, the patient's serum being absorbed with: (a) guinea pig kidney tissue; and (b) ox cells before titration of agglutinins. Guinea pig kidney absorbs both Forssman and serum-sickness antibodies; ox cells absorb serum-sickness and infectious mononucleosis antibodies, so that it is possible to distinguish between the three agglutinins.

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