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Figure 3. Improved oligo-cassette libraries amplified with a single fut8 P23 primer at different annealing temperatures (1. 68.0°C, 2. 67.3°C, 3. 65.9°C, 4. 63.7°C, 5. 60.6°C, 6. 58.5°C, 7. 57.0°C, 8. 56.0°C). The arrow indicates a band in lane 6 that is the desired 2.3-kb fragment that was confirmed by DNA sequences.

Figure 3. Improved oligo-cassette libraries amplified with a single fut8 P23 primer at different annealing temperatures (1. 68.0°C, 2. 67.3°C, 3. 65.9°C, 4. 63.7°C, 5. 60.6°C, 6. 58.5°C, 7. 57.0°C, 8. 56.0°C). The arrow indicates a band in lane 6 that is the desired 2.3-kb fragment that was confirmed by DNA sequences.

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