Chromatin immunoprecipitation ChIP assay

The ChIP assay was performed using salmon sperm DNA-conjugated protein A agarose (Upstate Cell Signaling Solutions, Charlottesville, VA, USA) according to the manufacturer's instructions. The immunoprecipitated DNA and the input DNA were analyzed by PCR using primers for the AFP promoter (direct primer; 5' -CTGAAGTGGTCTTTGTCCTTG-3' and reverse primer; 5'-ACTGTGAGCAGTAGCGCTG-3'). As a negative control, the coding region of the AFP gene was also amplified using primers (direct primer; 5 '-TCTGTGTTTCTGGATGAAATTTG-3' and reverse primer; 5'-ACATCGCCCTGTTTTCTTGG-3').

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