Culture Of Insulinoma Cells On Pancreas Sections

Yasuyuki Saito1'2, Satoshi Terada1, and Toshiaki Takezawa2

1Department of Applied Chemistry and Biotechnology,University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan; Laboratory of Animal Cell Biology, National Institute of Agrobiological Sciences, Ikenodai 2, Tsukuba, Ibaraki 305-0901, Japan

Abstract: Cell therapy using pancreatic islets is a promising lifelong treatment for type 1 diabetes mellitus. However, before cell therapy can be extensively used, there are some problems that need overcome. Of these, we focused on the need for improved cell function during in vitro culture. The sources of cells for cell therapy for diabetes are classified into two categories; primary cells isolated from a donor pancreas and established cell lines. The function of isolated primary cells decreases rapidly during in vitro culture and the function of established cell lines is too low for transplantation. To maintain and/or improve cell function during in vitro culture we designed TOSHI-substrata (substrata made of tissue / organ sections for histopathology), and investigated its effect on pancreatic cell lines derived from insulinomas. Before investigation, we estimated the influence of optimum cutting temperature (OCT) compound, which is used in preparation of the TOSHI-substrata, to confirm whether or not it significantly affects cell culture. We then cultured the cells on the TOSHI-substrata and found that they spontaneously aggregated; proliferation of the cells on the TOSHI-substrata was also slightly inferior to that on control glass slides, suggesting that culturing on pancreas sections might allow redifferentiation of the cell line or recovery of islet function.

Diabetes Sustenance

Diabetes Sustenance

Get All The Support And Guidance You Need To Be A Success At Dealing With Diabetes The Healthy Way. This Book Is One Of The Most Valuable Resources In The World When It Comes To Learning How Nutritional Supplements Can Control Sugar Levels.

Get My Free Ebook


Post a comment