Effect Of Il6 On Hybridoma Proliferation

We investigated the effect of IL-6 on the growth of two hybridoma cell lines. After culturing in 24-well plates with purified IL-6 for two days, cell number was counted. In the presence of IL-6, the growth of 2E3 cells was slightly accelerated, while that of its subclone 2E3-O adapted to serum-free medium was significantly suppressed (Fig.2). This result suggests that the IL-6-induced growth signal mechanism might differ in each cell line.

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