Expression of GSTCA150 WW domain

The system configuration for air bubbling is illustrated as in Figure 1A. The expression of GST-CA150 was monitored with sampling small aliquots of culture medium (Figure 1B). The increase in the production of GST-CA150 WW domain was saturated 2 hours after the induction in the case of shaking. But, the increase in the case of bubbling was saturated 5 hours after the induction. The total amount of production was approximately as much as 2-times of that of shaking. Monitored OD also showed the maximum value at this point, which was 3.5. This value was twice the value in the case of shaking. Thus, the increase in the amount of expression correlated with the total cell quantity of the culture.

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