Expression Of Recombinant Protein In Cho And HeLa Cells And Its Followup Using Egf Reporter Gene

V. Hendrick1, D. Ribeiro de Sousa1, A.R. dos Santos Pedregal1, C. Bassens1, P. Rigaux1, K. Sato2, K. Kotarsky3 and J. Werenne1

1 Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Faculty of Sciences, Université Libre de Bruxelles, Belgium; Lund University, Lund, Sweden; Tokyo Institute of Technology, Tokyo, Japan.

Abstract: The goal of this paper was to evaluate the potentialities of using chimeric EGFP-Photinus Luciferase reporter gene to follow the expression of the fusion protein by following the fluorescence for the development of recombinant protein production process and to evaluate the conditions in which the use of butyrate stimulation of protein production is effective.

Key words: EGFP; Photinus Luciferase; reporter gene; fusion protein; fluorescence microscopy and photometry; Cytofluorimetry; Butyrate stimulation; CHO; HeLa.

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