Measurements of peptidolytic activities

Goat anti-human lambda light chain antibody (Biosource International, Camarillo, CA) was used to fix the newly-expressed antibodies. The antibody diluted with phosphate-buffered saline (PBS) was added to a 96-well plate, and incubated for 2 h at 37°C. After washing with 0.05% Tween20-PBS (T-PBS), each well was blocked with 25% Block Ace (Dainihon) for 2 h at 37°C. After blocking reaction, the plate was washed with 0.05% T-PBS. One hundred ^.l of samples were added to a 96-well plate for 1 h at 37°C, the plate was washed with 0.05% T-PBS. The fluorescent peptide substrates were added and measured fluorescent intensities for 6 hours at 37°C.

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