Methods And Materials 21 Materials

The Caco-2 cell line was obtained from American Type Culture Collection (Rockville, MD, U.S.A.), and THP-1 cells were purchased from Health Science Research Resources Bank (Osaka, Japan). Dulbecco's modified Eagle's medium (DMEM) was purchased from Nissui Pharmaceuticals (Tokyo, Japan), and fetal calf serum (FCS) was from Sigma (St. Louis, MO, U.S.A.). Non-essential amino acids (NEAA) were purchased from Cosmobio (Tokyo, Japan). Recombinant human TNF-a, recombinant human IFN-y, recombinant human IL-1ß and recombinant human IL-6 were all purchased from Peprotech

(London, U.K.). Recombinant human IL-8, and biotinylated polyclonal antibodies against human TNF-a, IFN-y, IL-1ß, IL-6, and IL-8 were from Genzyme Techne (Cambridge, MA, U.S.A.).

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