Peptide synthesis

The ligand peptide with PL motif was synthesized by solid-phase peptide synthesis on a PSSM8 Peptide Synthesizer (Shimadzu, Kyoto). Rink amide AM resin (Novabiochem) and Fmoc-amino acids with protected side chains were used. Synthesized peptides were cleaved from the resin and deprotected in trifluoroacetate (TFA) in the presence of scavengers (5% water, 5% thioanisole, 3% ethylmethylsulfide, 2.5% ethandithiol, 2% thiophenol), and purified by reverse-phase chromatography with a preparative column ODS-AP303 (4.6 x 250 mm, YMC, Japan). Their molecular masses were also verified by MALDI-TOF-MS. The designed sequence of the ligand peptides was SPPAPPTPPPLPPP (from mouse formin).

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