Fucoidan was obtained from the Daiichi Sangyo Corporation (Osaka, Japan) as healthy functional food (Power fucoidan). The undiluted solution was neutralized to PH7.0 by 1 M NaOH. The Precipitation was removed by Centrifuge at 3500rpm for 15min. The supernatant was then sterilized by 0.2^m pore filter (Millipore, MA, USA) and stored in 4°C. 2',7'-dichlorofluorescin diacetate (DCFH-DA) was obtained from Sigma (MO). WST-1[2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo-phenyl)-2#-tetrazolium, monosodium salt] was purchased from Kishida (Osaka, Japan). Matrigel was purchased from Funakoshi (Tokyo, JAPAN). Total and Phospho-p38 Mitogen-activated protein kinase (MAPK) antibody was obtained from Cell Signaling Technology (MA).
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