Preparation of the enzymedigested fucoidan extract and reagents

Fucoidan was obtained from the Daiichi Sangyo Corporation (Osaka, Japan) as healthy functional food (Power fucoidan). The undiluted solution was neutralized to PH7.0 by 1 M NaOH. The Precipitation was removed by Centrifuge at 3500rpm for 15min. The supernatant was then sterilized by 0.2^m pore filter (Millipore, MA, USA) and stored in 4°C. 2',7'-dichlorofluorescin diacetate (DCFH-DA) was obtained from Sigma (MO). WST-1[2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo-phenyl)-2#-tetrazolium, monosodium salt] was purchased from Kishida (Osaka, Japan). Matrigel was purchased from Funakoshi (Tokyo, JAPAN). Total and Phospho-p38 Mitogen-activated protein kinase (MAPK) antibody was obtained from Cell Signaling Technology (MA).

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