Results And Discussion

Halki Diabetes Remedy

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The survey pointed out that in the first therapy period the percentage of responder (referring to blood sugar and HbAlc) of 73.8% or 236 patients is really very high considering such a short therapy time (in average 6 days).

The remaining 26.8% respectively 84 patients that did not respond to the active substance, didn't to all appearances stick to their diet plans or there are any other reasons for this state of affairs which are not discovered so far.

The reaction of the substantial parameters like blood sugar and HbAlc to consumption of reduced water was among the responder high significant positive. (Figures 1 and 2)

Figure 1. Suppressive effect of Nordenau water on blood sugar level in a short therapy period of 236 human diabetes patients-responder group, p-0, 000.
Figure 2. Suppressive effect of Nordenau water on HbAlc level of 236 human diabetes patients in a short therapy period-responder group, p-0, 000.

The random sample group of 50 patients with additional blood free oxygen radicals test (FORT) resulted in similar ratio of responder (74% or 37 patients) and non-responder 26% or 13 patients respectively. The comparison between decrease of the blood sugar and HbA1c average values with the downwards trend of reactive oxygen species concentration in the blood among responders pointed out causal connections between the changes of these substantial diabetes parameters and the fluctuation of reactive oxygen species in the blood of test persons. (Figures 3- 5)

Figure 3. Decrease of the blood sugar average value of the 37 responder of the random sample group.

Figure 3. Decrease of the blood sugar average value of the 37 responder of the random sample group.

Figure 4. Decrease of the HbA1c average value of 37 responder of the random sample group.

Figure 4. Decrease of the HbA1c average value of 37 responder of the random sample group.

On the other hand this clinical data can be considered as a hypothesis that the reduced water may be in a position to suppress the ROS concentration in the blood of diabetes patients and therefore to improve the diabetes relevant test parameters. Furthermore could be said that there are the causal connections between the variability of blood ROS level and the changes of diabetes relevant test parameters. Furthermore we investigated the effect of Nordenau water on cytotoxicity of alloxan, a type-1 diabetes inducer, using hamster pancreatic B cells. Alloxan produces reactive oxygen species (ROS), which damage

Figure 5. Decrease of free oxygen radicals concentration in blood of 37 responder of random sample group.

Figure 5. Decrease of free oxygen radicals concentration in blood of 37 responder of random sample group.

Figure 6.
Figure 8.
Figure 9.

pancreatic B cells. Nordenau water scavenged alloxan-induced intracellular ROS and enhanced the glucose-stimulated elevation of intracellular ATP level and the release of insulin, protecting B cells from alloxan-induced cytotoxicity. Nordenau water also significantly suppressed the elevation of blood sugar level in alloxan-induced type 1 diabetes mouse.

These results suggest that natural reduced water such as Nordenau water may be effective in improving the deficient secretion of insulin from B cells in type 1-and 2-diabetes mellitus. In view of the fact that reduced water shows in vitro a stimulatory effect on the glucose uptake by muscle and fat cells, similar to that of insulin, which can be also reproduced in animal experiments - the results of this clinical trial seem to have sufficient correlation with the above mentioned results of basic researches to be worthy of serious consideration.

4. CONCLUSION

RWs like ERW, Hita Tenryosui water and Nordenau water were all anti-oxidative waters that could scavenge intracellular ROS in hamster pancreatic B-cells, HIT-T15.

Alloxan induced lowering of viability, increase of cytosolic free Ca2+, increase of DNA fragmentation, decrease of intracellular ATP, and decrease of glucose-stimulated insulin release. RW could inhibit all those cytotoxic effects of alloxan, increasing markedly the glucose-stimulated increase of ATP levels and insulin release.

The clinical trials pointed out the causal connections between the changes of blood sugar and HbA1c values and the fluctuation of reactive oxygen species concentration in the blood of the tested persons.

5. REFERENCES

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Hanaoka K (2001) Antioxidant effects of reduced water produced by electrolysis of sodium chloride. J Appl Electrochem 31: 1307-1313.

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Shirahata S (2002) Reduced water for prevention of diseases. Animal Cell Technology: Basic & Applied Aspects, Volume 12, pp. 25-30, Kluwer Academic Publishers, the Netherlands.

Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, Otsubo K, Morisawa S and Katakura Y (1997) Electrolyzed-reduced water scavenge active oxygen species and protects DNA from oxidative damage. Biochem Biphys Res Commun 234:269-274.

Shirahata S, Nishimura T, Kabayama S, Aki D, Teruya K, Otsubo K, Morisawa S, Ishii Y, Gadek Z and Katakura Y (2001) Anti-oxidative water improves diabetes. Animal Cell Technology: From Target to Market (eds. Linder-Olsson E et al.), pp. 574-577, Kluwer Academic Publishers, the Netherlands.

Takasu N, Asawa T, Komiya I, Nagasawa Y and Yamada T (1991) Alloxan-induced DNA strand breaks in pancreatic islets: Evidence for H2O2 as an intermediate. J Biol Chem 266: 2112-2114.

Tashiro H (1999) Clinical examination of alkaline ion water. Abstract book of Symposium "Electrolyzed functional water in therapy" in 25th Meeting of Japanese Medical Society. pp. 6-7.

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