Sugar consumption and lactate production

The improvement of cell proliferation by RA was not found in the galactose-based or mannose-based medium (data not shown). In the fructose-based medium, fructose consumption was found only in the presence of RA (see Fig. 2), implying that RA activated fructose metabolism of hybridomas. When compared with sugar utilization between the glucose-based and fructose-based media supplemented with RA, the utilization of fructose was slower than that of glucose. In addition, lactate production in the fructose-based medium supplemented with RA was lower than that in the glucose-based medium supplemented with RA (data not shown). These findings correspond to the results of other investigators (Imamura et al., 1982; Petch et al., 1996).

AE6 cell line

BD9 cell line

AE6 cell line

1 2 3 4 5 Culture day

1001

BD9 cell line

1001

1 2 3 4 5 Culture day

Ctrl RA

1 2 3 4 5 Culture day

Figure 2. RA activated fructose metabolism of human hybridoma cell lines. Cells (1 x 105 cells/ml) were cultured in a fructose-based ITES-RPMI1640 medium supplemented with or without 10-7 M RA for 4 days. Control (Ctrl) received an equal amount of ethanol without RA. After 4 days, fructose concentrations in the medium were measured by using a fructose assay kit.

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