Trace Element Optimization Enhances Performance And Reproducibility Of Serumfree Medium

Scott J. Jacobia1, Robert W. Kenerson1, Lia D. Tescione1, Dale F. Gruber1, David W. Jayme2, Donald G. Munroe1, and Stephen F. Gorfien1

1Invitrogen Corporation, 3175 Staley Road, Grand Island, New York 14072, USA; Department of Biochemistry, Brigham Young University — Hawaii, 55-220 Kulanui Street, Laie, Hawaii 96762, USA

Abstract: Optimizing the quantity and composition of trace elements in the AEM (Adenovirus Expression Medium) formulation, resulted in an improved version of AEM, which is a serum-free, low protein, animal-origin free medium designed for adenovirus expression applications for PER.C6® and other mammalian cell types. The improved AEM formulation supports higher maximal viable cell growth than other commercially available serum-free media. Optimization of medium trace elements has been shown to support equivalent cell growth in production batches from 100 L to 1500 L. Adding a proprietary trace element mixture to lower performing lots of medium resulted in increased cell growth comparable to better performing lots also containing the mixture. Trace element optimization has been investigated in several additional Gibco® serumfree media. The resulting modifications can support maximal viable cell densities 1.5 to 3-fold greater than their predecessors. For example, HEK293 cell growth is supported with peak viable cell densities in excess of 4.0 x 106 viable cells/mL without a lag in cell growth during subsequent passages. Investigation continues to confirm biological productivity and to eliminate recombinant protein constituents and improve biological performance.

Key words: bioproduction; optimization; trace elements; PER.C6®; HEK293; CHO-S; HeLa.

PER.C6® is a registered trademark of Crucell N.V. Gibco® is a registered trademark of Invitrogen Corporation.

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