In 1900 Karl Landsteiner described the ABO blood grouping system and observed that individuals could be placed into different groups based on their blood type. This was the first step in the development of forensic haemogenetics. In 1915 Leone Lattes published a book describing the use of ABO typing to resolve a paternity case and by 1931 the absorption-inhibition ABO typing technique that became standard in forensic laboratories had been developed. Following on from this, numerous blood group markers and soluble blood serum protein markers were characterized and could be analysed in combination to produce highly discriminatory profiles. The serological techniques were a powerful tool but were limited in many forensic cases by the amount of biological material that was required to provide highly discriminating results. Proteins are also prone to degradation on exposure to the environment.
In the 1960s and 1970s, developments in molecular biology, including restriction enzymes, Sanger sequencing , and Southern blotting , enabled scientists to examine DNA sequences. By 1978, DNA polymorphisms could be detected using Southern blotting  and in 1980 the analysis of the first highly polymorphic locus was reported . It was not until September 1984 that Alec Jeffreys realized the potential forensic application of the variable number tandem repeat (VNTR) loci he had been studying [1, 12]. The technique developed by Jeffreys entailed
extracting DNA and cutting it with a restriction enzyme, before carrying out agarose gel electrophoresis, Southern blotting and probe hybridization to detect the polymorphic loci. The end result was a series of black bands on X-ray film (Figure 1.3). VNTR analysis was a powerful tool but suffered from several limitations: a relatively large amount of DNA was required; it would not work with degraded DNA; comparison between laboratories was difficult; and the analysis was time consuming.
A critical development in the history of forensic genetics came with the advent of a process that can amplify specific regions of DNA - the polymerase chain reaction (PCR) (see Chapter 5). The PCR process was conceptualised in 1983 by Kary Mullis, a chemist
Ladder Ladder Udder
Ladder Ladder Udder
working for the Cetus Corporation in the USA . The development of PCR has had a profound effect on all aspects of molecular biology including forensic genetics, and in recognition of the significance of the development of the PCR, Kary Mullis was awarded the Nobel Prize for Chemistry in 1993. The PCR increased the sensitivity of DNA analysis to the point where DNA profiles could be generated from just a few cells, reduced the time required to produce a profile, could be used with degraded DNA and allowed just about any polymorphism in the genome to be analysed. The first application of PCR in a forensic case involved the analysis of single nucleotide polymorphisms in the DQa locus  (see Chapter 12). This was soon followed by the analysis of short tandem repeats (STRs) which are currently the most commonly used genetic markers in forensic science (see Chapters 6 to 8). The rapid development of technology for analysing DNA includes advances in DNA extraction and quantification methodology, the development of commercial PCR based typing kits and equipment for detecting DNA polymorphisms.
In addition to technical advances, another important part of the development of DNA profiling that has had an impact on the whole field of forensic science is quality control. The admissibility of DNA evidence was seriously challenged in the USA in 1987 -'People v. Castro' ; this case and subsequent cases have resulted in increased levels of standardization and quality control in forensic genetics and other areas of forensic science. As a result, the accreditation of both laboratories and individuals is an increasingly important issue in forensic science. The combination of technical advances, high levels of standardization and quality control have led to forensic DNA analysis being recognized as a robust and reliable forensic tool worldwide.
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