Conclusions

The use of adenovirus vectors for gene therapy has placed increased demands upon the technology for production, purification, and characterization of virus particles. Some of the classic technology has been reexamined and improved. A new class of methods based upon column chromatography has added a powerful set of tools to this array. In large part, the chromatographic methods are based upon modes of chromatography and resins originally developed for protein purification. With proper consideration for the size and other characteristics of adenovirus particles column chromatography may be applied with considerable success. The past decade has witnessed rapid advances in this area. Column chromatography is now a preferred method for adenovirus purification because of its versatility and ability to purify large amounts of virus to a high state of purity while retaining biological activity.

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