■ The correct procurement of iliac, superior mesenteric, carotid and jugular vessels is crucial as complex vascular reconstructions might be required during the implantation. Therefore, the whole iliac axis down to the inguinal ligament must be taken.
■ The bile duct should be routinely rinsed by means of a small feeding tube through the cut end, as remaining bile salts precipitate and may damage the biliary epithelium. Flushing the biliary tree through the gallbladder cleans only the distal bile duct and thus is not enough!
■ Correct back-table bath temperature must be guaranteed. Warming up of the solution and direct contact with the ice can both be harmful and can easily be avoided by separating the allograft from the crushed ice through a cold saline buffer.
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