As most culture media used in research are used in small volumes, it is not necessary to make large volumes of media. Only small volumes of 10-50 ml are typically required. Therefore, it is easiest to prepare media from concentrated stock solutions. Media components are divided into like components with similar in-solution shelf-lives. For example, a stock A would usually contain salts and carbohydrates that are stable in solution for several months. However, for all media it is necessary to add the calcium chloride as a separate stock solution because this chemical can precipitate with other salts.
Individual stock solutions are prepared and can be filtered through a 0.2-mm filter and stored at 4°C. An example of stock groupings and shelf-lives are shown in Protocol 3.5.
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