Preparation of Stock Solutions

As most culture media used in research are used in small volumes, it is not necessary to make large volumes of media. Only small volumes of 10-50 ml are typically required. Therefore, it is easiest to prepare media from concentrated stock solutions. Media components are divided into like components with similar in-solution shelf-lives. For example, a stock A would usually contain salts and carbohydrates that are stable in solution for several months. However, for all media it is necessary to add the calcium chloride as a separate stock solution because this chemical can precipitate with other salts.

Individual stock solutions are prepared and can be filtered through a 0.2-mm filter and stored at 4°C. An example of stock groupings and shelf-lives are shown in Protocol 3.5.

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