Vid

Angiotensin II (AII) may be a proinflammatory molecule. The effect of AII on production of the chemokine RANTES was examined in cultured glomerular endothelial cells. A, Effects of AII on secretion of RANTES by cultured glomerular endothelial cells. AII markedly stimulated RANTES secretion. Of note is that AII-induced RANTES secretion was prevented by incubation with the AT2 receptor antagonists SCP-42112A (CGP) or PD 1231777 (PD) but not by the ATI receptor antagonist losartan (los). These finding suggest AT2 receptors mediate the increase in secretion of RANTES. B, Results of a chemotactic assay for human monocytes. Migration of monocytes was assessed using a modified Boyden chamber. Migration of monocytes was stimulated by conditioned medium from glomerular endothelial cells that were exposed to AII. This effect was blocked by incubation of the medium with an anti-RANTES antibody but not by control serum. The anti-RANTES antibody alone was also without effect, as was AII in the absence of conditioned media. The findings are consistent with the hypothesis that AII promotes glomerular inflammation by binding to AT2 receptors, promoting RANTES secretion and infiltration of inflammatory monocytes and macrophages. fg—femtograms. (From Wolf and coworkers [13]; with permission.)

Renin-angiotensin systems

Renin-angiotensin systems

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