Figure 116

Screening for anti-doubled-stranded DNA (dsDNA) antibodies using the Crithidia assay. The hemoflagellate Crithidia luciliae contains in its kinetoplast pure dsDNA, not complexed to proteins [3]. Serially diluted serum samples are added to the slide carrying Crithidia cells. Binding of antibodies is visualized by fluorescinated anti-immunoglobulin G antibodies. Antibodies to dsDNA are almost pathognomonic for systemic lupus erythematosus and therefore can be regarded as marker antibodies [4]. (From Klippel and Croft [5]; with permission.)

Test serum containing anti-dsDNA

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