Figure 1612

ATP depletion perturbs normal endoplasmic reticulum (ER) function. Because ATP and a proper redox environment are necessary for folding and assembly [20, 54, 63, 64] and ATP depletion alters ATP levels and the redox environment, the secretion of proteins is perturbed under these conditions. Here, Western blot analysis of the culture media from thyroid epithelial cells subjected to ATP depletion (ie, treatment with antimycin A, an inhibitor of oxidative phosphorylation) illustrates this point. A, Treatment with as little as 1|xM antimycin A for 1 hour completely blocks the secretion of thyroglobulin (Tg) from these cells.

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