Figure 1618

Three-dimensional extracellular matrix gel tubulogenesis model. Model of the three-dimensional gel culture system used to study the branching and tubulogenesis of renal epithelial cells. Analyzing the role of single factors (ie, extracellular matrix, growth factors, cell-signaling processes) involved in ureteric bud branching tubulo-genesis in the context of the developing embryonic kidney is an extremely daunting task, but a number of model systems have been devised that allow for such investigation [77, 79, 85]. The simplest model exploits the ability of isolated kidney epithelial cells suspended in gels composed of extracellular matrix proteins to form branching tubular structures in response to growth factors. For example, Madin-Darby canine kidney (MDCK) cells suspended in gels of type I collagen undergo branching tubulogenesis reminiscent of ureteric bud branching morphogenesis in vivo [77, 79]. Although the results obtained from such studies in vitro might not correlate directly with events in vivo, this simple, straightforward system allows one to easily manipulate individual components (eg, growth factors, extracellular matrix components) involved in the generation of branching epithelial tubules and has provided crucial insights into the potential roles that these various factors play in epithelial cell branching morphogenesis [77, 79, 84-87].

0 0

Post a comment