Figure 1619

An example of the branching tubulogenesis of renal epithelial cells cultured in three-dimensional extracellular matrix gels. Microdissected mouse embryonic kidneys (11.5 to 12.5 days) were cocultured with A, murine inner medullary collecting duct

(mIMCD) or, B, Madin-Darby canine kidney (MDCK) cells suspended in gels of rat-tail collagen (type I). Embryonic kidneys (EK) induced the formation of branching tubular structures in both mIMCD and MDCK cells after 48 hours of incubation at 37oC. EKs produce a number of growth factors, including hepatocyte growth factor, transforming growth factor-alpha, insulin-like growth factor, and transforming growth factor-^, which have been shown to effect tubulo-genic activity [86-93]. Interestingly, many of these same growth factors have been shown to be effective in the recovery of renal function after acute ischemic insult [21-30]. (From Barros et al. [87]; with permission.)

Pregnant SV40-transgenic mouse

Pregnant SV40-transgenic mouse

Isolate embryos

Dissect out embryonic kidney

Isolate metanephric mesenchyme

Isolate ureteric bud

Culture to obtain immortalized cells

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