Figure 1620

Development of cell lines derived from embryonic kidney. Flow chart of the establishment of ureteric bud and metanephric mesenchymal cell lines from day 11.5 mouse embryo. Although the results obtained from the analysis of kidney epithelial cells— Madin-Darby canine kidney (MDCK) or murine inner medullary collecting duct (mIMCD) seeded in three-dimensional extracellular matrix gels has been invaluable in furthering our understanding of the mechanisms of epithelial cell branching tubulogenesis, questions can be raised about the applicability to embryonic development of results using cells derived from terminally differentiated adult kidney epithelial cells [94]. Therefore, kidney epithelial cell lines have been established that appear to be derived from the ureteric bud and metanephric mesenchyme of the developing embryonic kidney of SV-40 transgenic mice [94, 95]. These mice have been used to establish a variety of "immortal" cell lines.

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