Figure 167

Madin-Darby canine kidney (MDCK) cell calcium switch. Insight into the molecular mechanisms involved in the assembly of tight junctions (that may be at least partly applicable to the ischemia-reperfusion setting) has been gained from the MDCK cell calcium switch model [43]. MDCK cells plated on a permeable support form a monolayer with all the characteristics of a tight, polarized transporting epithelium. Exposing such cell monolayers to conditions of low extracellular calcium (less than 5|xM) causes the cells to lose cell-cell contact and to "round up." Upon switching back to normal calcium media (1.8 mM), the cells reestablish cell-cell contact, intercellular junctions, and apical-basolateral polarity. These events are accompanied by profound changes in cell shape and reorganization of the actin cytoskeleton. (From Denker and Nigam [19]; with permission)

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