Figure 1726

Schematic representation of a differential display gel in which mRNA from kidneys is reverse-transcribed and polymerase chain reaction (PCR) amplified (see Figure 17-25). The PCR amplification is conducted in the presence of radioactive nucleotides. The cDNA fragments corresponding to the 3' end of the mRNA species are displayed by running them on a sequencing gel, followed by autoradiography. The arrows show bands corresponding to mRNA transcripts that are expressed differentially 1) in response to insulin-like growth factor (IGF-I) treatment and induction of ischemic injury; 2) in an IGF-I-dependent manner; 3) in response to induction of ischemic injury; and 4) to genes that are down-regulated after induction of ischemic injury. ARF—acute renal failure.

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

Get My Free Ebook


Post a comment