Figure 810

Scoring of complement-dependent cytotoxicity. In an effort to standardize interpretation of complement-dependent cytotoxicity (CDC) reactions, a uniform set of scoring criteria have been established. When most of the cells are alive, visually refractile on microscopic examination, a score of 1 is assigned. Conversely, when most of the cells are dead, a score of 8 is assigned. This method of interpretation for CDC reactions is universally used in cross-match testing, antibody screening, and antigen phenotyping for serologically defined HLA-A, -B, -C, -DR, and -DQ. (Adapted from Gebel and Lebeck [1]; with permission.)

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