Correct methodology for detecting circulating cryoglobulins. Cryoglobulins are immunoglobulins that precipitate reversibly from cooled serum.

Prewarm syringe, needle, and tubes at 37°C

Take 20 mL of whole blood and put it immediately at 37°C

Incubate for 2 h at 37°C to allow clotting

Centrifuge twice at 1700 g X 10 at 37°C to discard platelets and erythrocytes Cryoglobulins precipitate reversibly from cooled serum Keep serum at 4°C in a conical graduate tube Look at the serum after 7 d

Centrifuge serum at 400 g X 10 at 4°C and calculate the cryocrit as the percentage of packed cryoglobulins and serum ratio

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