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Electronics Repair Manuals Summary

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Results And Discussion

A schematic diagram and the representation of the in vitro coculture system used in this study are shown in Fig. 1. The two different types of cell used with this method were not in contact with each other, enabling the cross talk to be observed between the two types of cell via the soluble factors secreted from each cell line.

The Value of Animal Models

Normal motor behavior is the final product of massive neural computation performed by tissues with precise three-dimensional organizations and connectivity patterns. Similar to an electronics technician searching for a defective transistor on a circuit board, clinicians and scientists engaged in movement disorders research identify neuronal populations causally associated with specific forms of abnormal motor behavior. Studies of this type are a critical first step in defining targets for stereotactic neurosurgical procedures. Animal models have been crucial for identifying the subthalamic nucleus (STN), globus pallidus pars interna, and ventral intermediate nucleus of the thalamus as appropriate targets for deep brain stimulation in patients with Parkinson disease, dystonia, and essential tremor, respectively. In vivo studies have also been critical for characterizing the neuro-physiological underpinnings of spasticity and task-specific dystonias and, as a consequence, rehabilitation...

Actin Reorganization Adenovirus Internalization

Figure 1 Schematic diagram of adenovirus cell entry events. Virus attachment is mediated by fiber protein (black) association with CAR. Subsequent interaction of the penton base (light gray) with av integrins (dark gray) promotes Ad internalization. Integrin-mediated Ad internalization also requires the participation of several signaling molecules (c-Src, pi 30CAS, PI 3K, and Rho GTPAses) that mediate actin polymerization.

Solid State Dosimeters

Fig.(7.17) Schematic diagram representing the Implantation of p-type material into an n-type host. Highly mobile electrons will then migrate from n- to p-type and less mobile holes will migrate from p- to n-type. The migration will continue until the build-up of positive space charge in the n-type, and negative space charge in the p-type becomes large enough to prevent further movement. Fig.(7.17) Schematic diagram representing the Implantation of p-type material into an n-type host. Highly mobile electrons will then migrate from n- to p-type and less mobile holes will migrate from p- to n-type. The migration will continue until the build-up of positive space charge in the n-type, and negative space charge in the p-type becomes large enough to prevent further movement.

Destruction and Survival of Interstellar Material

Schematic diagram of the protosolar nebula. The positions of the Kuiper belt and Oort cloud are shown, together with the formation zones of comets comprising these two families Oort cloud (long period) comets are thought to form closer to the protostar than the Kuiper belt (short period) ones. Figure 3.1. Schematic diagram of the protosolar nebula. The positions of the Kuiper belt and Oort cloud are shown, together with the formation zones of comets comprising these two families Oort cloud (long period) comets are thought to form closer to the protostar than the Kuiper belt (short period) ones.

Some major ideas and unanswered questions

The origin of life remains a fascinating and elusive topic. We have even less evidence about it than we have about the origins of photosynthesis or eukaryotes or sex, yet it is the focus of more speculation and debate. It is not a single problem but a constellation of problems, none of which has been fully solved, and different people think of it in different ways. Some authors have focused on the prebiotic formation of organic molecules, or of proteins, or nucleic acids, or membrane-like structures. Others take the view that to explain the origin of life, it is sufficient to explain the origin of molecular self-replication. We suggest that the phrase origin of life should denote the formation of a system fitting the characterisation of livingness that we established earlier (see the schematic diagram at the start of chapter 10).

Late Gene Expression and Virus Assembly

Figure 6 Schematic of the adenovirus DNA packaging domain. (A) A schematic diagram of the left end of the adenovirus genome including the inverted terminal repeat (ITR), the packaging enhancer region (nts. 194 to 380), and the El A 5' flanking region. The packaging repeats (A1 through A7) are represented by arrows. The El A transcriptional start site is indicated by an arrow at nt 499. (B) The nucleotide sequence of the Ad5 packaging domain is shown. Numbers at the top correspond to nucleotides relative to the left end terminus. A repeats 1 through 7 are encircled. (C) The A repeat consensus sequence is shown.

Factors Influencing Cardiomyocyte Differentiation

Action potentials and cell contractions of cardiomyocytes differentiated from mouse embryonic stem cells (ESCs). (A) The protocol is shown to induce differentiation of cultured mouse ESCs (ES-D3 cell line) to cardiomyocytes. Left column is a schematic diagram of the hanging-drops method to differentiate ESCs to heart cells. Right column shows single ESCs in culture (white arrows in upper panel, X200), an embryoid body (red arrow in middle panel, X100), and a beating cluster of cardiac-like cells (yellow arrow in lower panel, X200) corresponding to the time periods (black arrows) in culture after withdrawal of leukemia inhibitory factor (LIF) from the conditioned culture medium. (B) Cardiac action potentials were observed in a spontaneously beating cell approx 10 d after differentiation of mouse ESCs. (C) Cell contractions of ESC-derived cardiomyocytes are shown. Cell shortenings were detected and recorded by the edge-detection method. The amplitude and beating rate of cell...

Photographic Album of Anorectal Malformations and the Sphincter Muscles

Anorectal Malformation

The prints have been traced and colored. The schematic diagrams are color-coded yellow for urinary structures, green for smooth muscle of the bowel, red for the levator complex, brown for external sphincter muscle, purple for genital organs, and blue for cartilage. The red spots on the schematic diagrams serve to orient the sections of each specimen. The blue dots on some diagrams indicate the course of a fistula or bowel lumen.

Antigenic crossreactivity

Fig. 7.2. (and opposite) (a) Schematic diagram of the theory and practice of the monoclonal antibody-based ELISA described in Rockey et al. (1991a). An individual well of an ELISA plate is depicted after each step in the assay. (1) The wells are coated with a primary monoclonal antibody (MAb 4D3) and the unbound sites on the plate are then blocked with a non-specific protein, bovine serum albumin (not shown). (2) Serial dilutions of a standard p57 preparation or dilutions of clinical samples are added. The primary antibody 4D3 binds to an amino-proximal epitope on p57, specifically retaining p57 but not other proteins in the sample. (3) A secondary biotinylated MAb (3H1) is used to recognize a specific epitope in the middle of p57. B, biotin. (4) MAb 3H1 is biotinylated, which is bound subsequently by an avidin-enzyme conjugate. (5) A chromogenic substrate is added and the colorimetric reaction is measured on an ELISA reader. (b) Photo of an ELISA plate after substrate addition. The...

The Intrabeam Machine and Surgical Technique

Source Intrabeam

Fig. 12.2 The Intrabeam x-ray source (middle) and applicators (left). The schematic diagram (right) shows how the target tissues are irradiated from within the breast and how the intrathoracic structures are protected with a thin shield Fig. 12.2 The Intrabeam x-ray source (middle) and applicators (left). The schematic diagram (right) shows how the target tissues are irradiated from within the breast and how the intrathoracic structures are protected with a thin shield

Automated And Semiautomated Dna Synthesis

Polypeptide Laboratory Mcgill

FIGURE 1.17 Schematic diagram of an early prototype DNA synthesizer used at McGill University in 1980. FIGURE 1.17 Schematic diagram of an early prototype DNA synthesizer used at McGill University in 1980. Early automated DNA synthesizers with chlorophosphite and phosphoramidite coupling chemistry used solid-phase supports packed into high-pressure columns similar to HPLC columns.98 This was, not surprisingly, because the early silica gel supports were based on HPLC media. It was a relatively simple matter to assemble a linear manifold of three-way valves to deliver the appropriate synthesis reagents and solvents to a high-pressure HPLC pump under some type of programmable control. The pump then forced the reagents through the synthesis column and into a waste receptacle. Addition of another valve on the outlet end of the column allowed reagents to be recycled through the synthesis column. A schematic diagram of an early semi-automated DNA RNA prototype synthesizer used by the author...

Physical Processes Affecting Chemistry

Schematic diagram of the protosolar nebula showing physical processes which affect chemistry. These include sources of ionisation (stellar X-rays, stellar UV, interstellar UV, cosmic rays, radionuclides), heating (viscous dissipation, stellar radiation) and transport (e.g. radial mixing, diffusion). Figure 3.3. Schematic diagram of the protosolar nebula showing physical processes which affect chemistry. These include sources of ionisation (stellar X-rays, stellar UV, interstellar UV, cosmic rays, radionuclides), heating (viscous dissipation, stellar radiation) and transport (e.g. radial mixing, diffusion).

Peri Infarct Depolarisations PIDS

Schematic diagram illustrating the concept of an ischaemic penumbra or boundary zone in experimental focal cerebral ischaemia in the cat brain, induced in this case by permanent occlusion of the right middle cerebral artery. The ectosylvian (EG), suprasylvian (SG) and marginal (MG) gyri lie at respectively increasing distances from the proximal Sylvian fissure. Directions of arterial inputs from the anterior and middle cerebral (MCA) arteries are indicated (posterior cerebral omitted for clarity). The heavily shaded area represents the core cortical territory associated with permanent MCA occlusion terminal depolarisation has occurred within an hour or less of occlusion and is irreversible except by early reperfusion. The lighter shaded area (penumbra) is the site of recurrent peri-infarct depolarisations originating at the edge of the core and propagating outwards into the penumbra (see text and Fig. 5). The square area represents the field of view in each panel of Fig. 5...

Cortical Spreading Depression

Schematic diagram illustrating current concepts of the role of astrocytes in cerebral perfusion and metabolism (adapted with permission from Tsacopoulos and Magistretti 55 Copyright 1996 by the Society for Neuroscience). Cerebral capillaries are extensively invested by astrocyte end feet, and extraction of glucose from blood to brain (probably the astrocyte compartment) is highly efficient (arrow 2). During activation, and especially in cortical spreading depression, the glycolytic pathway in astrocytes is upregulated, and the different kinetics of glial and neuronal lactate dehydrogenases favour net movement of lactate from astrocytes to neurons the (limited) brain glycogen pool is located in astrocytes. Under resting conditions, glycolysis in neurons may be sufficient to meet energy demands (arrow 1). Neurotransmitter glutamate released into the synaptic cleft is re-accumulated into astrocytes by high-affinity cotransport with Na+ ion, making use of the normal...

Amplification and attenuation of signals

The analogy between cellular signalling and electronic circuitry breaks down in one important respect. If A, B, C etc. in the schematic diagrams were electronic circuit components, there would normally be only one of each. But if they are signalling pathway intermediates this is not the case. A single activated receptor will usually activate many molecules of the type M Each M will activate several of type A, each of which will activate several of type B, and so on. Thus, a very tiny stimulus - the activation of a mere handful of receptors on the cell surface - can induce a very large response in a very short time.

Electrochemical biosensors

Figure 4.22 Schematic diagram of a Held effect transistor. The silicon-silicon dioxide system exhibits good semiconductor characteristics for use in FETs. The free charge carrier concentration, and hence the conductivity, of silicon can be increased by doping with impurities such as boron. This results in p-type silicon, the 'p' describing the presence of excess positive mobile charges present. Silicon can also be doped with other impurities to form n-type silicon with an excess of negative mobile charges. Figure 4.22 Schematic diagram of a Held effect transistor. The silicon-silicon dioxide system exhibits good semiconductor characteristics for use in FETs. The free charge carrier concentration, and hence the conductivity, of silicon can be increased by doping with impurities such as boron. This results in p-type silicon, the 'p' describing the presence of excess positive mobile charges present. Silicon can also be doped with other impurities to form n-type silicon with an excess of...

Figure 213

Schematic diagram of neural connections important in circulatory control. Although the system is bilaterally symmetric, afferent fibers are shown to the left and efferent fibers to the right. Sympathetic fibers are shown as solid lines and parasympathetic fibers as dashed lines. The heart receives both sympathetic and parasympathetic innervation. Sympathetic fibers lead to vasoconstriction and renal sodium chloride retention. X indicates the vagus nerve IX indicates glossopharyngeal. (From Korner 25 with permission.)

Figure 214

Mechanisms of glomerular injury in hypertension and progressive renal failure. This schematic diagram summarizes the general mechanisms by which disturbances linked to elevated arterial pressure in patients with parenchymal renal disease may lead to further tissue injury. Hemodynamic changes lead to increased glomerular perfusion pressures, whereas local activation of growth factors, angiotensin, and probably several other factors both worsen peripheral resistance and increase tissue fibrotic mechanisms. (From Smith and Dunn 1 .)

Figure 143

The mesangium regulates single-nephron glomerular filtration rate (SNGFR) by altering the glomerular ultrafiltration coefficient (Kf). This schematic diagram demonstrates the anatomic relationship between glomerular capillary loops and the mesangium. The mesangium is surrounded by capillary loops. Mesangial cells (M) are specialized pericytes with contractile elements that can respond to vasoactive hormones. Contraction of mesangium can close and prevent perfusion of anatomically associated glomerular capillary loops. This decreases the surface area available for glomerular filtration and reduces the glomerular ultrafiltration coefficient.

Figure 1034

Functional renal alterations in preeclampsia. The functional consequences of glomerular endotheliosis and of the hormonal alterations in preeclampsia are summarized in this schematic diagram of the nephron in preeclampsia. Suppression of the renin-angiotensin system occurs, probably in response to vasoconstriction and elevated blood pressure. The glomerular lesion leads to proteinuria, which may be heavy. Renal hemodynamic changes include modest decreases in the glomerular filtration rate (GFR) and renal blood flow (RBF). Decreased sodium and uric acid excretion may be caused by increased proximal tubular reabsorption. The mechanism for the marked hypocalciuria is not known.

Results

Fig. 23.6 Schematic diagram of the trocar passing from the peritoneum between the two bellies of the pubococcygeus muscle into the abdomen. A grasper is advanced through the port to grasp the distal end of the dissected rectum. The rectum is then pulled down through the perineum trailing the trocar Fig. 23.6 Schematic diagram of the trocar passing from the peritoneum between the two bellies of the pubococcygeus muscle into the abdomen. A grasper is advanced through the port to grasp the distal end of the dissected rectum. The rectum is then pulled down through the perineum trailing the trocar

Anatomy

Schematic diagram illustrating the axonal connectivity pattern between the nose and the MOB. The OE in mice is divided into four zones (zones I through IV) that are defined by the expression of odorant receptors. Olfactory sensory neurons in a given zone of the epithelium project to glomeruli located in a corresponding zone (zones I through IV) of the MOB. Axons of sensory neurons expressing the same odorant receptor (red or dark blue) converge to only a few defined glomeruli. NC Neocortex AOB accessory olfactory bulb. Reprinted (abstracted excerpted) with permission from Mori K, Nagao H, Yoshihara Y (1999). The olfactory bulb coding and processing of odor molecule information. Science 286 (5440) 711-715. Copyright (1999) AAAS Fig. 1. Schematic diagram illustrating the axonal connectivity pattern between the nose and the MOB. The OE in mice is divided into four zones (zones I through IV) that are defined by the expression of odorant receptors. Olfactory sensory neurons in a...

Endocrine Regulation

Schematic diagram of the human androgen receptor gene. Black areas transactivation domains P proline directed phosphorylated Ser residues HSP heat shock protein pKC protein kinase C pKA protein kinase A MAPK mitogen-activated protein kinase DNA-Dep Kin DNA-dependent kinase Ser-Pro serine-proline directed kinase Cas-Kin II casein kinase II. Adapted from Wiener et al.,164 MacLean et al.,176 Blok et al.,175 and Zhou et al.186 FIGURE 14-5. Schematic diagram of the human androgen receptor gene. Black areas transactivation domains P proline directed phosphorylated Ser residues HSP heat shock protein pKC protein kinase C pKA protein kinase A MAPK mitogen-activated protein kinase DNA-Dep Kin DNA-dependent kinase Ser-Pro serine-proline directed kinase Cas-Kin II casein kinase II. Adapted from Wiener et al.,164 MacLean et al.,176 Blok et al.,175 and Zhou et al.186

Figure 196

Modalities for anticoagulation for continuous renal replacement therapy. While systemic heparin is the anticoagulant most commonly used for dialysis, other modalities are available. The utilization of these modalities is largely influenced by prevailing local experience. Schematic diagrams for heparin, A, and citrate, B, anticoagulation techniques for continuous renal replacement therapy (CRRT). A schematic of heparin and regional citrate anticoagulation for CRRT techniques. Regional citrate anticoagulation minimizes the major complication of bleeding associated with heparin, but it requires monitoring of ionized calcium. It is now well-recognized that the longevity of pumped or nonpumped CRRT circuits is influenced by maintaining the filtration fraction at less than 20 . Nonpumped circuits (CAVH HD HDF) have a decrease in efficacy over time related to a decrease in blood flow (BFR), whereas in pumped circuits (CVVH HD HDF) blood flow is maintained however, the constant pressure...

Tick syst Host Tick

Schematic diagram of possible transmission routes and blockades of B. burgdorferi s.l. from infected vector ticks to hosts and recipient ticks. An asterisk means the tick or host is infected. (1) Permissive system with horizontal and vertical transmission through the cycle. Example B. afzelii-infected tick feeding on a rodent. (2) Non-permissive system. No horizontal transmission from the infected tick (midgut infection) to the host and no vertical transmission to the next developmental stage of the tick (i.e. elimination of midgut infection in vector). Example tick infected with B. afzelii feeding on an avian host or deer. (3) Semi-permissive system. Horizontal transmission to the host by systemically infected vector ticks and possible vertical transmission through the moult, but no further transmission to recipient ticks. Example ticks systemically infected with B. garinii (e.g. OspA serotypes 5, 6) may infect rodents. However, rodents carrying such strains do not infect...

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