Figure 6. Gauging T-cell expansion. Qualitative and quantitative assessment of T-cells. Flow-cytometric determination of individual TCR VB families (I). TCR-VB-CDR3 analysis was performed (II). This landscape picture can be 'corrected' by implementing TCR VB frequencies of individual VB families as defined by flow cytometry (III). Note the difference between the 'quality' (II) and the 'quality-quantity' analysis (III). T-cells represent freshly harvested CD4+ TIL.
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